Phospho mtor ser2448

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August undefined, 2024

WebAnti-phospho-mTOR (Ser2448) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling, Glucose/Glycogen Metabolism, Insulin/Energy Signaling, Adhesion (CAMs) Gene Symbol: FRAP1, RAPT1, mTOR, FRAP, … WebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor.

ab168538 – Kit mTOR (pSer2448) ELISA - Abcam

WebPrinciple of Assay. This mTOR (phospho Ser2448) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2448) and the effects that certain stimulation conditions have on mTOR (phospho Ser2448) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2448) concentration is achieved by an indirect … WebPolyclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1872 publications. Validated for Western Blotting, Simple Western™. Available in 2 sizes. Highly specific and … truman french

Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb #5536

WebNov 7, 2015 · Antibodies against total phospho-AMPK(Thr172), total phospho-ACC(Ser79), total phospho-p53(Ser15), phospho-Raptor (Ser722/ Ser792), total phospho-mTOR(Ser2448), total phospho-p70S6K(Thr389), total phospho4EBP-1 (Thr 37/46), total phospho-Akt(Ser 473), LC3 I/II β-actinwere obtained fromCell Signaling Technology, … WebJul 8, 2005 · Previous studies have shown that mTOR is a direct substrate for the AKT kinase and identified Ser-2448 as the AKT target site in mTOR. In this study, we … WebJan 1, 2024 · Starting on day 15, mice were treated three times weekly with 1 mg/tumor Mito-MGN administered via an intratumoral injection in a 50 μL volume, and the control mice were injected with a vehicle of 20% ethanol in phosphate buffered saline. philippine archipelago formation

Human and Mouse Phospho-mTOR (S2448) ELISA RayBiotech

Dysregulated metabolic pathways in age-related macular ... - Nature

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 213 publications. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and … WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) … philippine archipelago major landformsWebthe complex. Phosphorylation of mTOR at Ser2448 is carried out directly by AKT kinase as well as p70S6 kinase acting as a feedback signal. Phosphorylation at this site is a biomarker for the activation state of the PI-3 kinase pathway as well as the activation status of mTOR. Activation of mTOR leads to phosphorylation of PRAS40, philippine architectureboards reddit

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Phospho mtor ser2448

WebThe Phospho -mTOR (Ser2448) Assay is a sandwich immunoassay (Figure 1). MSD provides a plate that has been pre-coated with capture antibody for total mTOR . The user adds the sample and a solution containing the detection antibody—anti-phospho-mTOR (Ser2448) conjugated with an electrochemiluminescent WebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, …

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WebmTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX132803 WB Image Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody … WebThe Phospho-mTor (Ser2448) assay measures mTor when phosphorylated at Ser2448. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-mTor (Ser2448) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor.

WebPhospho-mTOR (S2448) and Total mTOR ELISA Kit (ab279869) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, …

WebPhospho-mTOR (Ser2448) Antibody (25-9718-42) in Flow Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 and CD28 Functional … WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation.

Web上海康朗生物科技有限公司主要致力于“Anti-Phospho-mTOR (Ser2448)抗体”的生产销售。多年的“Anti-Phospho-mTOR (Ser2448)抗体”生产与销售的经验，与各行业新老用户建立了 …

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … philippine archipelago meaningWeb斯氏毛滴虫pcr检测试剂盒厂家上海联祖生物相关产品:28s rrnp检测试剂盒实验流程1.实验前标准品、试剂及样本的准... philippine archipelago vectorWebJan 9, 2024 · Both mTORC1 and mTORC2 are regulated by phosphorylation, complex formation and localization. So far, the role of p70S6K-mediated mTOR S2448 phosphorylation has not been investigated in detail. Here, we report that endogenous mTOR phosphorylated at S2448 binds to both, raptor and rictor. truman frontierWebPhospho-mTOR (Ser2448) (49F9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human … philippine architecture firmsWebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts … truman futchWebApr 10, 2024 · RayBio® Human and Mouse Phospho-mTOR (Ser2448) ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 in cell lysate samples. MANUAL / SDS $521.00 Contact Us for Bulk Request Skip to the end of the images gallery Skip to the beginning of the images gallery Product Description Specifications Risk-Free … truman from the truman showWebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for … truman from ww2