Phosphinothricin ppt
WebPublished: October 1999 A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats Sonriza Rasco-Gaunt, Amanda Riley, Paul Lazzeri & Pilar Barcelo … WebPost sterilization, seeds were spread out onto plates that contained standard Arabidopsis growth medium (half-strength Murashige and Skoog (MS) salts) which was supplemented with the selective agent, phosphinothricin (PPT), at a concentration of 10 mg/L.
Phosphinothricin ppt
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WebSep 12, 2024 · The transformed calli were selected on the media containing increasing concentrations of phosphinothricin (PPT, 3 mg/L and 6 mg/L). After shooting and rooting, the calli were cultured in the regeneration media for 2 weeks in light at 25 °C, and transferred to soil after 2-day adaption in water. WebPhosphinothricin (PPT) is the active ingredient in bialaphos, which specifically inhibits glutamine synthetase in land plants. We isolated a novel PPT-resistant gene from a soil …
WebApr 1, 2024 · Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of … WebJan 1, 1995 · The bialaphos resistance gene (bar) is part of the biosynthesis pathway of bialaphos 6.The strains Streptomyces hygroscopicus and Streptomyces …
WebApr 24, 2024 · Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. WebA fatty acid photodecarboxylase (CvFAP) based photobiocatalytic deracemization of phosphinothricin (PPT) was developed. The engineered CvFAP enabled the kinetic …
WebSep 12, 2014 · 此可读框架通过组成型启动子CaMV35S启动，携带PPT乙酰基转移酶(PAT)基因作为选择标记基因，并将构建的表达载体导入根癌农杆菌EHA105。确定烟草转化的潮霉素临界筛选浓度为50mg，头孢噻肟钠的抑菌浓度为200mg。
WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am … how many watts is 600 maWebPhosphinothricin is a broad-spectrum herbicide that acts as a competitive inhibitor of glutamine synthetase. Glutamine synthetase plays an important role in plant nitrogen … how many watts is 50 kwWeb1994; Wan and Lemaux 1994). L-Phosphinothricin (PPT), or the ammonium salt, glufosinate, is the active compound of the non-selective herbicide Basta. L-Phos phinothricin is a glutamate analogue that inhibits glutamine synthetase (Manderscheid and Wild 1986). Glutamine synthetase catalyses an ATP-dependent incor how many watts is 580 lumensWebDescription DL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®, Glufosinate Ammonium, PPT, 2-amino-4- (hydroxymethylphosphinyl) butanoic acid Product Information Shipping and Storage Related Documents how many watts is 6000 btuWebMar 9, 2024 · This chapter describes a protocol for protoplast transformation and plant regeneration for major forage and turf grass species, including tall fescue, red fescue, meadow fescue, perennial ryegrass, and Italian ryegrass. Embryogenic calli induced from caryopsis were used to establish embryogenic cell suspension cultures. how many watts is 5200 btuWebAug 16, 2007 · The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase ( pat) gene as a selectable marker and 5.0 mg l −1 of bialaphos as the selective agent. how many watts is 5kvaWebPhosphinothricin acetyltransferase ( EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin … how many watts is 600 volts