WebDec 31, 2012 · It was a high-copy number plasmid with ampicillin resistance gene, P L P R promoter, cIts857 gene, multiple cloning sites (MCS), Shine-Dalgarno (SD) sequence, and two strong transcription terminators, and suitable for all kinds of hosts. 2.2. Expression plasmid construction WebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble …

Evidence for the Double-Strand Break Repair Model of

Webogy), is rec+ (A kil335 cIts857 P3). The kil mutation was isolated by H. Greer (10). BIK1199 is a A-resistant mutant from M5361. BIK1189 is M5361 cured of A. The recAl strains DH1 and DH5 (11) and plasmid pIK43 were described previously (12). The recombination rate of DHI(pIK43) to Neo+ cells is as low as 4 x 10-7 per cell generation (12). WebApr 17, 2013 · Primers were designed to amplify the entire sequences of pBV-DsRed and pBV-OPH, except the cIts857 gene which encodes the repressor protein for transcriptional control in plasmid pBV220 (primers used were no. 5 and no. 6, see Table 1). The amplified fragment was then self-circularized to construct the plasmids pL-DsRed and pL-OPH. crypt or niche

原核表达载体的重要调控元件-上海中洪博元

WebXX RN [3] RC pJLf201 from phage fd terminator & lambda, cIts857 gene/pR/pL RA McCarthy J.E., Schairer H.U., Sebald W.; RT "Translational initiation frequency of atp genes from Escherichia RT coli: identification of an intercistronic sequence that enhances RT translation"; RL EMBO J. 4:519-526(1985). Web1．启动子. 启动子是dna链上一段能与 rna聚合酶 结合并起始rna合成的序列，它是基因表达不可缺少的重要调控序列。 没有启动子，基因就不能转录。由于细菌rna聚合酶不能识别真核基因的启动子，因此原核表达载体所用的启动子必须是原核启动子。 WebThis was isolated as spontaneous mutant of a lambda Nam7 Nam53 cIts857 prophage. Mycoplasma contamination Not detected Technical information ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. cryptoenvoy